Effects of Harvest and Fertilization Frequency on Protein Yield and Extractability From Flood-Tolerant Perennial Grasses Cultivated on a fen Peatland

Paludiculture, and in particular the cultivation of perennial grasses as biomass feedstock for green biorefineries, may be an economic environmentally sustainable option agricultural peatlands temperate regions. However, optimal quality protein extraction from flood-tolerant is largely unknown. The aim this study was to define combined effect harvest fertilization frequency, with one five annual cuts, on yield extractability tall fescue (TF) reed canary grass (RCG), cultivated fen peatland Denmark.The content fractions determined according Cornell Net Carbohydrate Protein System (CNCPS). We assessed by lab-scale biorefinery techniques using a screw-press followed acid precipitation true protein. two methods were compared correlate potential extractable yields actual outputs. found highest crude (CP) cut treatments, 13.4 15.6 t dry matter (DM) ha −1 year , containing 2.9–3.4 CP TF RCG, respectively. neutral-extractable (fractions B 1 2 ) 1.1 1.5 representing 39% - 45% (RCG) total CP. Using biorefining techniques, we able precipitate up 2.2 DM concentrate, Significant correlations between found, distinct relationship CNCPS + indicating suitability indicator yields. Biomass not significantly improved beyond cuts. timing frequencies affected plant maturity consequently contents concentrate conclude that RCG are promising feedstocks biorefineries due high biomass, CP, yields, highlight grasses, wet peatlands, enhanced valorisation common utilisation bioenergy.